Along with building slender and longer span structures, vibration serviceability becomes more important considerations in bridge design and maintenance. In this study, vibration serviceability and deflection limit for long span cable bridges are investigated using real-time and long-term monitoring data such as accelerations and displacements of bridges. Various criteria are investigated including limit values in the code and other research results. Korean Bridge Design Code (Limit State Design) specifies L/400 and L/350 for cable stayed and suspension bridges, respectively. Barker and Saadeghvaziri suggested deflection limit based on natural frequency, acceleration limit and vehicle speed. Various human comfort criteria on vibration are also investigated including ISO standards. Real-time and long-term monitoring data are analyzed and compared with these. Probability of exceedance are calculated for each criterion.  The results of this study are expected to be useful reference for the design, the proper planning and deflection review of the long span cable bridges around the world.